[Histonet] RE: cytoprep

[Tony Henwood (SCHN)]

The saline re-hydration works well as long as smears are not allowed to dry too long before rehydration.
I have found that air-drying longer than 2 hours decreases the effectiveness of the procedure.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Thursday, 31 January 2013 5:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cytoprep

Hi to all-
I am continuing to have a problem with Pap stains done by hand:

1.       We rehydrate 25 seconds in saline and straight into alcohol.  Thicker areas are ok, but thinner areas appear to be air dried......

2.       Red cell ghosts are NOT clearing
I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have.

Nancy Schmitt HT, MLT
Dubuque, IA





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