[Histonet] RE: cytoprep

Tony Henwood (SCHN) tony.henwood <@t> health.nsw.gov.au
Wed Jan 30 16:04:39 CST 2013

The saline re-hydration works well as long as smears are not allowed to dry too long before rehydration.
I have found that air-drying longer than 2 hours decreases the effectiveness of the procedure.

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Thursday, 31 January 2013 5:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cytoprep

Hi to all-
I am continuing to have a problem with Pap stains done by hand:

1.       We rehydrate 25 seconds in saline and straight into alcohol.  Thicker areas are ok, but thinner areas appear to be air dried......

2.       Red cell ghosts are NOT clearing
I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have.

Nancy Schmitt HT, MLT
Dubuque, IA

NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you.

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

More information about the Histonet mailing list