[Histonet] RE: cytoprep

[Tony Henwood (SCHN)]

Joe,

The method I believe Nancy is using is based on the ability of saline to re-hydrate air-dried smears prior to PAP staining:

Chan and Kung (1988) "Rehydration of air-dried smears with normal saline. Application in fine-needle aspiration cytologic examination".Am J Clin Pathol 89:30-34.

Ng WF, Choi FB, Cheung LL, Wu C, Leung CF, Ng CS "Rehydration of air-dried smears with normal saline. Application in fluid cytology" .Acta Cytolog (1994) 38(1):56-64.

Jane E. Dahlstrom, Janice Holdsworth, Mark L. Bassett, and Sanjiv Jain, "Rehydration of Air-Dried Smears: An Alternative Method for Cytologic Analysis of Exfoliative Cells" Acta Cytol 1999;43:214-217

Vinod B. Shidham, Bal Kampalath, and James England "Routine Air Drying of All Smears Prepared During Fine Needle Aspiration and Intraoperative Cytology Studies: An Opportunity to Practice a Unified Protocol Offering the Flexibility of Choosing a Variety of Staining Methods" Acta Cytol 2001; 45:60-68

Ganesan Sivaraman and Krishnan R. Iyengar "Rehydrated Air-Dried Pap Smears as an Alternative to Wet-Fixed Smears" Acta Cytol 2002; 46:713-717

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: Joe W. Walker, Jr. [mailto:joewalker <@t> rrmc.org] 
Sent: Friday, 1 February 2013 2:16 AM
To: Tony Henwood (SCHN); 'Nancy Schmitt'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: cytoprep

I assume that these are conventional Pap tests where the material is smeared onto the slide.  I am curious why the slides are air dried upon collection instead of applying a spray fixative to the slide.  If these are non-gyn slides from a brushing or FNA, then we might need to have a different discussion.

As someone with numerous years experience with cytoprep, I would need more information to help trouble shoot what might be the real issue and to offer suggestions.

Cheers,

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
NEW EMAIL: joewalker <@t> rrmc.org
www.rrmc.org

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN)
Sent: Wednesday, January 30, 2013 5:05 PM
To: 'Nancy Schmitt'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: cytoprep

The saline re-hydration works well as long as smears are not allowed to dry too long before rehydration.
I have found that air-drying longer than 2 hours decreases the effectiveness of the procedure.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Thursday, 31 January 2013 5:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cytoprep

Hi to all-
I am continuing to have a problem with Pap stains done by hand:

1.       We rehydrate 25 seconds in saline and straight into alcohol.  Thicker areas are ok, but thinner areas appear to be air dried......

2.       Red cell ghosts are NOT clearing
I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have.

Nancy Schmitt HT, MLT
Dubuque, IA





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