[Histonet] RE: cytoprep
Ian R Bernard
ibernard <@t> uab.edu
Wed Jan 30 13:25:38 CST 2013
Our rehydration time is 30-45 secs. Then into 95% alcohol.
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Wednesday, January 30, 2013 12:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cytoprep
Hi to all-
I am continuing to have a problem with Pap stains done by hand:
1. We rehydrate 25 seconds in saline and straight into alcohol. Thicker areas are ok, but thinner areas appear to be air dried......
2. Red cell ghosts are NOT clearing
I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have.
Nancy Schmitt HT, MLT
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