[Histonet] RE: cytoprep

Ian R Bernard ibernard <@t> uab.edu
Wed Jan 30 13:25:38 CST 2013

Our rehydration time is 30-45 secs. Then into 95% alcohol.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Wednesday, January 30, 2013 12:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cytoprep

Hi to all-
I am continuing to have a problem with Pap stains done by hand:

1.       We rehydrate 25 seconds in saline and straight into alcohol.  Thicker areas are ok, but thinner areas appear to be air dried......

2.       Red cell ghosts are NOT clearing
I believe there are quite a few Histology labs also performing cytoprep work and I will appreciate any thoughts you may have.

Nancy Schmitt HT, MLT
Dubuque, IA

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