[Histonet] RE: SDH methods for muscles

[Tony Henwood (SCHN)]

I have found that the major problem with most of these enzymehistochemical stains is that the sections should not be allowed to sit too long at room temperature before staining. Either stain immediately on sectioning or store at -70oC.
The enzymes tend to deteriorate quite rapidly at room temp.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Celebre Julia
Sent: Saturday, 26 January 2013 6:01 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] SDH methods for muscles

I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem.
If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get.



Julia Celebre   Sr MLT
Anatomic Pathology
Hamilton General Hospital
905-527-4322  ext 46179


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