[Histonet] Which fixative for eyes

[Lucie Guernsey]

I'm curious about this as well.

We work with mice and rats in our lab and whenever we need eyes (corneas,
more specifically), we immersion fix them whole in Bouin's solution
overnight at 4C then transfer to 70% EtOH until we're ready to excise the
corneas, process and embed into paraffin. We've found that we get nice
half-moon sections with good looking stromal and epithelial layers this way
compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged
corneas). We then perform IHC.

Would love to know if there's a better way, even though this seems to work
for us.

Thanks,
Lucie

Lucie Guernsey
UCSD
lguernsey <@t> ucsd.edu



On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro <
LouroP <@t> princeton.huntingdon.com> wrote:

> Hello to the eye experts,
>
>
>
> I am currently working on a large animal (LA) eye project which will
> give me the flexibility of experimenting with different fixatives.
>
> I am currently using Modified Davidson's solution (48 hours) followed by
> a 10% NBF solution prior to trimming the eyes, embedding , microtomy and
> H&E staining.
>
>
>
> After literature research, I found that different labs are using:
>
>
>
> Modified Davidson's solution
>
> Davidson's solution
>
> 10% NBF
>
> Gluteraldehyde
>
> Some places inject the eye with fixative before submerging the eye
> itself.
>
>
>
> I wanted to get some ideas / preferences from the experts out there so I
> can cover my entire basis before finalizing my conclusions.
>
>
>
> Thanks in advance to all,
>
>
>
> Pedro Louro
>
> Group Leader Histology / IHC
>
>
>
>
>
>
>
>
>
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