[Histonet] Which fixative for eyes

Louro, Pedro LouroP <@t> Princeton.Huntingdon.com
Wed Jan 16 12:32:15 CST 2013

Hello to the eye experts,


I am currently working on a large animal (LA) eye project which will
give me the flexibility of experimenting with different fixatives.

I am currently using Modified Davidson's solution (48 hours) followed by
a 10% NBF solution prior to trimming the eyes, embedding , microtomy and
H&E staining.


After literature research, I found that different labs are using:


Modified Davidson's solution

Davidson's solution

10% NBF


Some places inject the eye with fixative before submerging the eye


I wanted to get some ideas / preferences from the experts out there so I
can cover my entire basis before finalizing my conclusions.


Thanks in advance to all,


Pedro Louro

Group Leader Histology / IHC




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