[Histonet] Which fixative for eyes

[Louro, Pedro]

Hello to the eye experts,

 

I am currently working on a large animal (LA) eye project which will
give me the flexibility of experimenting with different fixatives.

I am currently using Modified Davidson's solution (48 hours) followed by
a 10% NBF solution prior to trimming the eyes, embedding , microtomy and
H&E staining.

 

After literature research, I found that different labs are using:

 

Modified Davidson's solution

Davidson's solution

10% NBF

Gluteraldehyde

Some places inject the eye with fixative before submerging the eye
itself.

 

I wanted to get some ideas / preferences from the experts out there so I
can cover my entire basis before finalizing my conclusions.

 

Thanks in advance to all,

 

Pedro Louro

Group Leader Histology / IHC

 

 

 


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