[Histonet] Xylene Free Processing?

Sarah Dysart sdysart <@t> mirnarx.com
Wed Jan 9 14:48:06 CST 2013

FYI, I've tried ClearRite, and while I guess it technically works...it's a giant pain to work with because you have to extend out your times.  I also found that it screws with Eosin because if it gets moisture in it (which you can't see); the water bleaches out the Eosin.
Just my two cents worth =)

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, January 09, 2013 2:46 PM
To: Jones, Laura; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene Free Processing?

Please go to http://www.histosearch.com/rene.html and you will find my articles on the sibject.
René J.

From: "Jones, Laura" <lpjones <@t> srhs-pa.org>
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, January 9, 2013 2:37 PM
Subject: [Histonet] Xylene Free Processing?

I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute.  Our processor was down recently, and a friendly local lab processed our tissue this way for us.  Of course, the Pathologists loved it.  The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin.

What are your feelings concerning immunos?  Overall cost?  Any other thoughts?

Thanks in advance!


Sharon Regional Health System is the area's largest hospital
and provider of health care services. Visit us online at
http://www.sharonregional.com for a complete listing of our
services, primary care physicians and specialists, and satellite locations.

Confidentiality Note: This message is intended for use
only by the individual or entity to which it is addressed
and may contain information that is privileged,
confidential, and exempt from disclosure under applicable
law. If the reader of this message is not the intended
recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution or
copying of this communication is strictly prohibited. If
you have received this communication in error, please
contact the sender immediately and destroy the material in
its entirety, whether electronic or hard copy. Thank you.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list