[Histonet] Xylene Free Processing?

[Sarah Dysart]

FYI, I've tried ClearRite, and while I guess it technically works...it's a giant pain to work with because you have to extend out your times.  I also found that it screws with Eosin because if it gets moisture in it (which you can't see); the water bleaches out the Eosin.
Just my two cents worth =)

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, January 09, 2013 2:46 PM
To: Jones, Laura; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene Free Processing?

Please go to http://www.histosearch.com/rene.html and you will find my articles on the sibject.
René J.

From: "Jones, Laura" <lpjones <@t> srhs-pa.org>
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, January 9, 2013 2:37 PM
Subject: [Histonet] Xylene Free Processing?

I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute.  Our processor was down recently, and a friendly local lab processed our tissue this way for us.  Of course, the Pathologists loved it.  The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin.

What are your feelings concerning immunos?  Overall cost?  Any other thoughts?

Thanks in advance!

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