[Histonet] IHC Buffer (wash)
Sandra Cheasty
cheastys <@t> svm.vetmed.wisc.edu
Wed Dec 4 16:10:21 CST 2013
Hello everyone,
Got your Christmas shopping done? (Didn't think so...)
Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some at VWR and FISHER (no shipping charges for our lab) that are 0.5% and 1% Tween 20 washing buffer in PBS.
Which percentage is best for IHC on a Lab Vision stainer using a polymer detection kit? As usual, your input is appreciated.
Sandy
P.S. I know I can make up my own from scratch, just not interested in doing that at the moment...
Sandra Cheasty
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine
608 263-1680 (Office)
608 263-1055 (Lab)
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