[Histonet] RE: IHC Buffer (wash)

[Goins, Tresa]

Try ScyTek - The ready-made price is nearly as cheap as I can make it myself.  http://www.scytek.com/default.asp 

Tresa




Tresa Goins
Histopathology Section Supervisor
Montana Veterinary Diagnostic Lab




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty
Sent: Wednesday, December 04, 2013 3:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Buffer (wash)

Hello everyone,

                Got your Christmas shopping done? (Didn't think so...) 

Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some at VWR and FISHER (no shipping charges for our lab) that are 0.5% and 1% Tween 20 washing buffer in PBS.

                Which percentage is best for IHC on a Lab Vision stainer using a polymer detection kit? As usual, your input is appreciated.

Sandy

 

P.S. I know I can make up my own from scratch, just not interested in doing that at the moment...

 

Sandra Cheasty

Histology & Necropsy Supervisor

UW-Madison, School of Veterinary Medicine

608 263-1680 (Office)

608 263-1055 (Lab)

 

 

 



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