[Histonet] rat's hind paw skin

[Leila Etemadi]

Hi,

First I would like to say thanks a lot to you and also Carl who gave me some helpful tips. Then I would like to ask if you have any practical tips when I want to pick up the skin from perfused animal. I need those special tricks which are not normally mentioned in the articles!, I am going to collect them nest week, kind of stressed since I don’t want to lose the tissues because of clumsy handling!, I you have any tips or link which can help me…?!?

Thanks a lottttt…

Cheers,

Leila

On 03 Dec 2013, at 01:27, Barry Rittman <barryrittman <@t> gmail.com> wrote:

> Hi Leila
> As no one seems to have responded to your email I will add a few items.
> I used to do some work on wound healing on mouse footpads so circumstances
> are the same.
> If you are looking for melanin routine fixation and processing should be
> finem, melanin will remain.
> Only thing is that the epidermis is tough and there is not much dermis in
> this region.
> Need to make sure that you are careful in cutting sections or the epidermis
> will separate.
> Hope this helps
> if you need any more details please email me.
> Barry
> 
> 
> 
> On Mon, Dec 2, 2013 at 3:44 AM, Leila Etemadi <leila.etemadi <@t> med.lu.se>wrote:
> 
>> Hello every body,
>> 
>> First I would like to thank you all for your great feedbacks and attention
>> which is such a big improvement in my work, Thank you all!
>> 
>> Then, I was wonder if any one has experience on this tissue: rat hind paw
>> skin ( which is hairless).
>> 
>> Any inputs will be appreciated, whether is about any specific source where
>> I can find technical information about fixation procedure..., or about
>> sectioning,staining procedure…
>> 
>> I am looking for melanin pigments and have no clue if it is possible to
>> see it there or not?!?
>> 
>> Thanks in advance,
>> 
>> Cheers
>> 
>> Leila
>> 
>> 
>> 
>> 
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