[Histonet] rat's hind paw skin

[Barry Rittman]

Hi Leila
As no one seems to have responded to your email I will add a few items.
I used to do some work on wound healing on mouse footpads so circumstances
are the same.
If you are looking for melanin routine fixation and processing should be
finem, melanin will remain.
Only thing is that the epidermis is tough and there is not much dermis in
this region.
Need to make sure that you are careful in cutting sections or the epidermis
will separate.
Hope this helps
if you need any more details please email me.
Barry



On Mon, Dec 2, 2013 at 3:44 AM, Leila Etemadi <leila.etemadi <@t> med.lu.se>wrote:

> Hello every body,
>
> First I would like to thank you all for your great feedbacks and attention
> which is such a big improvement in my work, Thank you all!
>
> Then, I was wonder if any one has experience on this tissue: rat hind paw
> skin ( which is hairless).
>
> Any inputs will be appreciated, whether is about any specific source where
> I can find technical information about fixation procedure..., or about
> sectioning,staining procedure…
>
>  I am looking for melanin pigments and have no clue if it is possible to
> see it there or not?!?
>
> Thanks in advance,
>
> Cheers
>
> Leila
>
>
>
>
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