[Histonet] RE: Embedding cells in Paraffin

[Morken, Timothy]

Mike,

For EM work we fix, scrape the plate into a microtube, centrifuge at 1800  rpm for 10 min and then embed in Histogel or agar. Processing after that is as usual. They look fine by EM. I'm sure they would look fine by paraffin processing as well.

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Tighe
Sent: Monday, December 02, 2013 9:06 AM
To: histonet <@t> lists.utsouthwestern.edu (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] Embedding cells in Paraffin

Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair.



Thanks for any ideas!
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