[Histonet] Purple/Blue haze on ISH

[Bauer, Karen L.]

Hi all... sending out for a colleague...

After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides.  Not all of them, but some and it seems to be consistent.  No problems before Vantage.  They were using the longer labels from Ventana for labeling prior to implementation, but now with Vantage, the slide labels are smaller.  That's the only real difference from before and after.  Protocols have not changed and they've checked everything else... buffers and such.

Any ideas on why this would happen?

Thank you,

Karen

Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen <@t> mayo.edu<mailto:bauer.karen <@t> mayo.edu> | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org<http://www.mayoclinichealthsystem.org/>