[Histonet] Embedding cells in Paraffin

[joelle weaver]

I spun down the cultured cells and then used a product called histogel. It kept them protected through the VIP, paraffin embedded like cell block "buttons" and was able to cut regular paraffin sections. I believe that I purchased the histogel from American Mastertech. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: mtighe <@t> trudeauinstitute.org
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 2 Dec 2013 17:06:24 +0000
> Subject: [Histonet] Embedding cells in Paraffin
> 
> Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair.
> 
> 
> 
> Thanks for any ideas!
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