[Histonet] RE: Purple/Blue haze on ISH

[Obregon, Cecilia]

Karen,

We experienced a similar problem with the ISHs at our laboratory a few months ago with the Kappa, Lambda, Eber and HPV. Our pathologists were complaining of a blue crystal-like precipitate on all ISH slides.  

Our Ventana Technical Specialist recommended that we try the NO ALCOHOL dehydrating procedure. (The same one we currently use for the Red-Alkaline Phosphatase detection). Once you remove the slides from the Ultra or XT rinse throughly in soapy water and put in the 60 Degrees oven for 15 minutes. Dehydrate in the Symphony using a NO DEHYDRATION coverslip program. 

We have noticed a signficant decrease in this precipitate using this procedure. Hope if works for you too. 

Thank you,

Cecilia M. Obregon
Memorial Regional Hospital 
3501 Johnson Street
Hollywood, FL 33021
Ph: 954-265-5317

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Bauer, Karen L. [Bauer.Karen <@t> mayo.edu]
Sent: Monday, December 02, 2013 12:08 PM
To: 'histonet'
Subject: [Histonet] Purple/Blue haze on ISH

Hi all... sending out for a colleague...

After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides.  Not all of them, but some and it seems to be consistent.  No problems before Vantage.  They were using the longer labels from Ventana for labeling prior to implementation, but now with Vantage, the slide labels are smaller.  That's the only real difference from before and after.  Protocols have not changed and they've checked everything else... buffers and such.

Any ideas on why this would happen?

Thank you,

Karen

Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen <@t> mayo.edu<mailto:bauer.karen <@t> mayo.edu> | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org<http://www.mayoclinichealthsystem.org/>


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