[Histonet] Immunofluorescence on paraffin sections

[Martha Ward-Pathology]

We do quite a number of renal biopsies and sometimes the frozen tissue is not sufficient for the immunofluorescent panel and we have to use paraffin sections for our fluorescence staining.   It has never really worked all that well and so we are looking for alternative methods.   
We get all our FITC labeled antibodies from Dako and would like to use them on our Bond III stainer, not for immunofluorescence but for immunoperoxidase staining.   Has anyone out in Histoland tried this on the Bond, and if so, would you share your procedure with me?

Thanks in advance for any help you can give me.

 
Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mward <@t> wakehealth.edu