[Histonet] IHC after EDTA decalcifying

[Bea DeBrosse-Serra]

Hi Colleen, 

We use "ChelatorCal" from BBC Biochemical, which contains EDTA. It is very gentle and retains antigenicity for IHC stain. We really like it. 

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Colleen Forster
Sent: Thursday, August 15, 2013 4:59 PM
To: Histonet
Subject: [Histonet] IHC after EDTA decalcifying

Hello fellow histonetters,

Can someone who has worked with decalcifyied bone tell me if EDTA interferes with IHC staining? I was under the impression it did not but cannot get staining in mouse femurs that I have decaled in EDTA. I have used both the steamer and the decloaker for retrieval and in the human tonsil the stain is great.

Any suggestions......

Thanks in advance!

Colleen Forster
U of MN

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