[Histonet] IHC after EDTA decalcifying

[koellingr <@t> comcast.net]

Could I ask exactly what you are looking for in mouse femur and human tonsil the tonsil  I assume is a control?  For what?  Whatever you are looking for is obviously in human tonsil.  Are you positive it is in murine femur.  I used to stain for 15-20 targets in EDTA decaled mouse femurs but my control was also a mouse. 



Ray 

Research Scientist 

Comparative Medicine 

University of Washington 


----- Original Message -----


From: "Colleen Forster" <cforster <@t> umn.edu> 
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, August 15, 2013 4:58:35 PM 
Subject: [Histonet] IHC after EDTA decalcifying 

Hello fellow histonetters, 

Can someone who has worked with decalcifyied bone tell me if EDTA 
interferes with IHC staining? I was under the impression it did not but 
cannot get staining in mouse femurs that I have decaled in EDTA. I have 
used both the steamer and the decloaker for retrieval and in the human 
tonsil the stain is great. 

Any suggestions...... 

Thanks in advance! 

Colleen Forster 
U of MN 

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