[Histonet] IHC after EDTA decalcifying

[Colleen Forster]

Hello fellow histonetters,

Can someone who has worked with decalcifyied bone tell me if EDTA 
interferes with IHC staining? I was under the impression it did not but 
cannot get staining in mouse femurs that I have decaled in EDTA. I have 
used both the steamer and the decloaker for retrieval and in the human 
tonsil the stain is great.

Any suggestions......

Thanks in advance!

Colleen Forster
U of MN