[Histonet] Embedding problem

[Benjamin]

Plastic or metal molds?

Sent from my iPhone

On Aug 1, 2013, at 11:26 AM, Tim Wheelock <twheelock <@t> mclean.harvard.edu> wrote:

> Hi Everyone:
> 
> When I paraffin embed brain tissue, I gentle push down on all regions (especially the edges) of the specimen(s).
> It seems that all of the areas of the specimen (or each of several specimens) lie flat on the bottom of the mold.
> This should ensure that I can quickly obtain a full face when trimming, with a minimum loss of tissue.
> 
> However, I have found that when I remove the solidified blocks from the mold, parts of the specimen, especially the cortical edges of the tissue, have lifted up from the bottom of the mold.
> I either have to re-embed the tissue, or trim through more tissue than I would like to obtain a full face for sectioning.
> 
> Has anyone had this problem?
> How do people avoid it?
> 
> Thank you,
> 
> Tim Wheelock
> Harvard Brain Bank
> McLean Hospital
> Belmont, MA
> 
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