[Histonet] Embedding problems-part 2

[Tim Wheelock]

Hi again Everyone:

Thank you for the suggestions.
I use the flat end of an old microtome orientation screw as a tamper.
I hold down the various parts of the specimen for around 5 seconds each, 
but I cannot hold down all areas of the specimen at once, due to the 
small size of the orientation  screw.
I have found that since brain tissue is so heterogeneous, certain parts 
are more suseptible to lifting than others, and I hold those down longer.
After I embed the specimen, I put it on the freezing plate, embed a 
couple of more blocks, then top off the first block with a bit more 
paraffin, and so forth.
Possibly the heat of the extra bit of wax is causing the lifting?

Thanks again

Tim Wheelock