[Histonet] softening agent for tissues embedded in paraffin blocks

[Rene J Buesa]

Your problem is not finding a "softener" but to decalcify your tissues correctly. Usually histotechs rub decalcifying solutions over the exposed area of the block but that wil affect the quality of the sections so, again, decalcify properly your tissues and you will not have any needs to make any additional "softening".
René J.


________________________________
From: Jose Luis Palazon Fernandez <jluis.palazon <@t> icman.csic.es>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Wednesday, September 26, 2012 5:56 AM
Subject: [Histonet] softening agent for tissues embedded in paraffin blocks


Dear List-members

I'm trying to cut some fish muscle samples (along with skin and some bone tissues) embedded in paraffin (fixed in Bouin's fixative and decalcified with EDTA). I find it very difficult to cut these blocks and I would like to know, based in your experience, what tissue softening agent do you recomend to solve this problem. Many thanks in advance

greetings,

José Luis



Dr. José Luis Palazón Fernández
Instituto de Investigaciones Científicas
Universidad de Oriente
Boca del Rio-Isla Margarita-Venezuela
Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC
Campus universitario Rio San Pedro, 11510, Puerto Real, Cádiz, España
email: jluis.palazon <@t> icman.csic.es; jose.palazon <@t> ne.udo.edu.ve
tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100
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