[Histonet] softening agent for tissues embedded in paraffin blocks

[Jose Luis Palazon Fernandez]

Dear List-members

I'm trying to cut some fish muscle samples (along with skin and some bone tissues) embedded in paraffin (fixed in Bouin's fixative and decalcified with EDTA). I find it very difficult to cut these blocks and I would like to know, based in your experience, what tissue softening agent do you recomend to solve this problem. Many thanks in advance

greetings,

José Luis



Dr. José Luis Palazón Fernández
Instituto de Investigaciones Científicas
Universidad de Oriente
Boca del Rio-Isla Margarita-Venezuela
Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC
Campus universitario Rio San Pedro, 11510, Puerto Real, Cádiz, España
email: jluis.palazon <@t> icman.csic.es; jose.palazon <@t> ne.udo.edu.ve
tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100