[Histonet] Rat Brain Serial Sections

[Erin Sarricks]

Hi Histonet-

I have to serial section rat brain slices through the entire block (5
blocks total per brain) for stereology.  Since it is for stereology, I
obviously need to minimize tissue loss while sectioning.  Does anyone
have any experience with using ammonium hydroxide to help with the
dryness?  Would you recommend soaking the block in ammonium hydroxide
or wiping the surface of the block with it between sections?  Also,
what dilution would you use?  Thanks in advance for your help!

Erin Sarricks, HT (ASCP)
Histology Team Leader
USAMRICD Comparative Pathology Branch