[Histonet] Looking for an IHC expert!
Broome, Michelle
michelle.broome <@t> novartis.com
Thu Sep 13 12:24:38 CDT 2012
Hello Histonetters!
Our preclinical lab is seeking an experienced IHC technician for an open position located in Cambridge, MA. Please email your resume to me directly if you are interested!
Scientist I to assist Novartis researchers with coordination and performance of pathology related projects and training of personnel as necessary. Specific activities include performing (with minimal or no supervision) routine and specialized necropsy (e.g. blood and bone marrow smear preparation, whole body perfusion, dissection of tissue and organ subsites, recognition and preservation of gross lesions, tissue collection for RNA/DNA and protein analysis, timed tissue collection, etc.); histology activities such as trimming, tissue processing, embedding, and microtomy, including cryotomy of frozen tissues. Expertise in routine and special staining techniques, immunohistochemistry, in-situ hybridization, and preparation of tissues and slides for electron microscopy are mandatory skills for this position. Other activities include assisting with quality control and archiving activities, maintenance of inventory of core laboratory supplies, and utilization of slide scanning equipment and image analysis software. The Scientist I will collect, verify and maintain study data and measurements, communicate results in an accurate and timely manner; will develop and validate new methodologies and validate laboratory instrumentation as needed. Leadership skills for training and instructing technicians or scientists in day-to-day operations and laboratory scheduling and resource allocation will be required.
Scientist I will display high level of competence in use of all laboratory equipment necessary to perform tasks as described above and to perform maintenance and troubleshooting when required.
Minimum requirements:
* HT certification or equivalent, or AS/AAS degree with 4-6 years experience, or Bachelor's
degree + 8, or Masters + 6 years experience.
* Documented ability to be self-motivated while making strong team-oriented contributions.
* Strong personal and team-oriented time management skills
* Computer skills in Microsoft windows-based office software.
* Demonstrated experience in the required area, and competency in all technical procedures
* Demonstrated understanding of relevant scientific and technical aspects
* Ability to supervise other scientists when necessary.
Preferred qualifications:
* HT or HTL certification
* Ventana expertise
Michelle Broome, MBA, HTL(ASCP)
Laboratory Manager
PCS Discovery and Investigative Pathology
Novartis Institutes for BioMedical
Research, Inc.
100 Technology Square
611-7104A
Cambridge, MA 02139
USA
Phone +1 617 8717477
Fax +1 617 8714931
michelle.broome <@t> novartis.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, September 13, 2012 1:02 PM
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Subject: Histonet Digest, Vol 106, Issue 15
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Today's Topics:
1. IHC lead (Webb, Dorothy L)
2. Re: IHC lead (Rene J Buesa)
3. RE: air drying special stain slides rather than
(Tony Henwood (SCHN))
4. RE: RE: air drying special stain slides rather than
(Tony Henwood (SCHN))
5. Re: RE: air drying special stain slides rather than
(E. Wayne Johnson)
6. voice recognition and synoptic reporting (Hutton, Allison)
7. Re: voice recognition and synoptic reporting (William Chappell)
8. RE: voice recognition and synoptic reporting (Michael Mihalik)
9. Sakura glas coverslipper and mounting media types (Cathy Crumpton)
----------------------------------------------------------------------
Message: 1
Date: Wed, 12 Sep 2012 13:17:02 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] IHC lead
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<65365F35C0F2EF4D846EC3CA73E49C4301DAA5E1CC0E <@t> HPEMX3.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"
What criteria does everyone use to hire for working in your IHC department? I am looking for feedback as to what qualifications are expected for IHC techs as well as qualifications and expectations of the "lead" in IHC. Also, what title do you have for the "lead" in IHC, technical specialist, coordinator, lead, etc???
Thanks ahead of time for your information! We are trying to reinvent the position and want to make it more like a community standard (community meaning the world of histology)!!
________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0
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Message: 2
Date: Wed, 12 Sep 2012 12:59:32 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] IHC lead
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1347479972.30506.YahooMailNeo <@t> web121403.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I always looked for a HTL (ASCP) certified and the title was Senior Histotechnologist Ren? J.
________________________________
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, September 12, 2012 2:17 PM
Subject: [Histonet] IHC lead
What criteria does everyone use to hire for working in your IHC department?? I am looking for feedback as to what qualifications are expected for IHC techs as well as qualifications and expectations of the "lead" in IHC.? Also, what title do you have for the "lead" in IHC, technical specialist, coordinator, lead, etc???
Thanks ahead of time for your information!? We are trying to reinvent the position and want to make it more like a community standard (community meaning the world of histology)!!
? ________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________
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Message: 3
Date: Thu, 13 Sep 2012 00:32:11 +0000
From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
Subject: [Histonet] RE: air drying special stain slides rather than
To: "'Mayer,Toysha N'" <TNMayer <@t> mdanderson.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6D6BD1DE8A5571489398B392A38A71579D1E982D <@t> xmdb02.nch.kids>
Content-Type: text/plain; charset="us-ascii"
We have routinely dried slides prior to coverslipping. We have found that ethanol or acetone rinsing after staining shortens the drying time (in fact we have used acetone to dehydrate MGG and DiffQuik stained smears, which must be kept away from alcohols, prior to coverslipping).
Our automatic coverslipper uses a very runny xylene based mountant so we do not need to rinse in xylene prior to coverslipping.
One paper we did describes the detergent de-waxing aspect and a study currently in preparation applies the technique to fungal staining:
Henwood A (2012) "The application of heated detergent dewaxing and rehydration to immunohistochemistry" Biotechnic & Histochemistry 87(1): 46-50.
It will depend on the staining method used as to whether you can use alcohol, acetone or heat-assisted drying prior to coverslipping, but, dare I say, nearly all stains can be treated thus.
Whoops, I forgot about the Oil Red O stains for fats, Oh well I did say "nearly all"!!
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Wednesday, 12 September 2012 1:42 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: air drying special stain slides rather than
Ooh, great question for my students next semester.
Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain.
Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide.
There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion.
Toysha N. Mayer, MBA, HT (ASCP)
Instructor, Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer <@t> mdanderson.org
Message: 16
Date: Tue, 11 Sep 2012 10:32:08 -0400
From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] air drying special stain slides rather than
dehydrate and clear
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DCFD9E6A390E294AAF3A2561CD32E5C417A90529 <@t> ckhamail1.ckha.on.ca>
Content-Type: text/plain; charset="us-ascii"
I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip.
Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution.
I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process.
Diana
------------------------------
Message: 17
Date: Tue, 11 Sep 2012 07:52:05 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] air drying special stain slides rather than
dehydrate and clear
To: Diana McCaig <dmccaig <@t> ckha.on.ca>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1347375125.72189.YahooMailNeo <@t> web121405.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Diana:
The most simple answer to your question is: "Because that is the way it has been done for more than 150 years".
The second question would be: "Is it necessary?" and the short answer to this question is: NO!!!
As a matter of fact, one of the steps I have developed to totally eliminate xylene from the histology lab refers to the "clearing" of stained sections, not only "special stains" (the so called HC and IHC) but the routine as well (the H&E).
Now, the "secret" to a successful drying of the stained slides is NOT to let them air dry because that will take not only too much time, but you can never be sure if the section is completely dry and if you add the mounting medium to a not completely dried section, you will have transparency problems.
The correct way of doing that is by drying the stained sections during 5 minutes at 60?C in an oven.
Under separate cover I am sending you something I published about your question and other aspects of how to completely eliminate xylene from ALL steps in the histology laboratory.
Ren? J.
________________________________
From: Diana McCaig <dmccaig <@t> ckha.on.ca>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, September 11, 2012 10:32 AM
Subject: [Histonet] air drying special stain slides rather than dehydrate and clear
I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip.
Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping.? Reason given is that the counterstain gets washed out.? Wouldn't adjusting the times be a better resolution.
I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process.
Diana
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
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------------------------------
Message: 4
Date: Thu, 13 Sep 2012 00:41:55 +0000
From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
Subject: RE: [Histonet] RE: air drying special stain slides rather
than
To: "'Diana McCaig'" <dmccaig <@t> ckha.on.ca>, "E. Wayne Johnson"
<ewj <@t> pigsqq.org>, Rene J Buesa <rjbuesa <@t> yahoo.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Mayer, Toysha N"
<TNMayer <@t> mdanderson.org>
Message-ID: <6D6BD1DE8A5571489398B392A38A71579D1E9889 <@t> xmdb02.nch.kids>
Content-Type: text/plain; charset="utf-8"
Yep
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Wednesday, 12 September 2012 3:23 AM
To: E. Wayne Johnson; Rene J Buesa
Cc: histonet <@t> lists.utsouthwestern.edu; Mayer, Toysha N
Subject: RE: [Histonet] RE: air drying special stain slides rather than
Would this work for auto cover slipping (tape film)if they were set in the xylene reservoir prior to cover slipping?
Diana
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson
Sent: September-11-12 1:15 PM
To: Rene J Buesa
Cc: 'histonet <@t> lists.utsouthwestern.edu'; Mayer, Toysha N
Subject: Re: [Histonet] RE: air drying special stain slides rather than
I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration.
I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me.
I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven.
What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok?
Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us.
EWJohnson
Enruikang Ag Tech
Beijing.
On 9/12/2012 12:01 AM, Rene J Buesa wrote:
> Toysha:
> Perhaps you have not oven dried stained slides before, and that explains some of your comments, like:
> 1- if the stained slides are completely dried, the "miscibility" you
> point out is not an issues, because there is nothing to mix with;
> 2- if you dehydrate ??? clear the stained sections that will take
> about
> 15 minutes per group of up to 25 slides, or even more depending on the
> protocol used in your automated stainer, but if your group of slides
> in their rack are placed in an oven at 60??C for 5 minutes it will
> just that, 5 minutes reducing the usual TAT for each staining
> procedure;
> 3- any oven can accommodate more than 100 stained slides in their
> racks and the TAT is shortened by oven drying, no matter how many
> slides you are working with;
> 4- I really do not know where you can find that "extreme heat" can
> affect the tissue sections. All tissue sections are fixed ???
> processed ??? dried (usually at the same 60??C before staining) ???
> stained and an additional step at 60??C to dry before cover-slipping
> is just that, an additional step at 60??C
> 5- The so called "Lean" technologies do not refer to staining only,
> they have to do with the whole work-flow and an additional drying step
> at 60??C cannot affect in a negative way to the work-flow
> 6- after staining you will oven dry the sections.
> I think you should try the method instead.
> Ren?? J.
>
>
> ________________________________
> From: "Mayer,Toysha N"<TNMayer <@t> mdanderson.org>
> To:
> "'histonet <@t> lists.utsouthwestern.edu'"<histonet <@t> lists.utsouthwestern.ed
> u>
> Sent: Tuesday, September 11, 2012 11:41 AM
> Subject: [Histonet] RE: air drying special stain slides rather than
>
>
> Ooh, great question for my students next semester.
> Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain.
>
> Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide.
>
> There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion.
>
>
> Toysha N. Mayer, MBA, HT (ASCP)
> Instructor, Education Coordinator
> Program in Histotechnology
> School of Health Professions
> MD Anderson Cancer Center
> (713) 563-3481
> tnmayer <@t> mdanderson.org
>
>
>
>
> Message: 16
> Date: Tue, 11 Sep 2012 10:32:08 -0400
> From: "Diana McCaig"<dmccaig <@t> ckha.on.ca>
> Subject: [Histonet] air drying special stain slides rather than
> dehydrate and clear
> To:<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DCFD9E6A390E294AAF3A2561CD32E5C417A90529 <@t> ckhamail1.ckha.on.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip.
>
>
>
> Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution.
>
>
>
> I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process.
>
>
>
> Diana
>
>
>
> ------------------------------
>
> Message: 17
> Date: Tue, 11 Sep 2012 07:52:05 -0700 (PDT)
> From: Rene J Buesa<rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] air drying special stain slides rather than
> dehydrate and clear
> To: Diana McCaig<dmccaig <@t> ckha.on.ca>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1347375125.72189.YahooMailNeo <@t> web121405.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Diana:
> The most simple answer to your question is: "Because that is the way it has been done for more than 150 years".
> The second question would be: "Is it necessary?" and the short answer to this question is: NO!!!
> As a matter of fact, one of the steps I have developed to totally eliminate xylene from the histology lab refers to the "clearing" of stained sections, not only "special stains" (the so called HC and IHC) but the routine as well (the H&E).
> Now, the "secret" to a successful drying of the stained slides is NOT to let them air dry because that will take not only too much time, but you can never be sure if the section is completely dry and if you add the mounting medium to a not completely dried section, you will have transparency problems.
> The correct way of doing that is by drying the stained sections during 5 minutes at 60?C in an oven.
> Under separate cover I am sending you something I published about your question and other aspects of how to completely eliminate xylene from ALL steps in the histology laboratory.
> Ren? J.
>
>
> ________________________________
> From: Diana McCaig<dmccaig <@t> ckha.on.ca>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Tuesday, September 11, 2012 10:32 AM
> Subject: [Histonet] air drying special stain slides rather than
> dehydrate and clear
>
> I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip.
>
>
>
> Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping.? Reason given is that the counterstain gets washed out.? Wouldn't adjusting the times be a better resolution.
>
>
>
> I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process.
>
>
>
> Diana
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
_______________________________________________
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This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
*********************************************************************************
------------------------------
Message: 5
Date: Thu, 13 Sep 2012 09:21:12 +0800
From: "E. Wayne Johnson" <ewj <@t> pigsqq.org>
Subject: Re: [Histonet] RE: air drying special stain slides rather
than
To: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, 'Diana McCaig'
<dmccaig <@t> ckha.on.ca>, "Mayer, Toysha N" <TNMayer <@t> mdanderson.org>
Message-ID: <50513508.6030708 <@t> pigsqq.org>
Content-Type: text/plain; charset=UTF-8; format=flowed
Interestingly, I showed the results to a couple of colleagues.
One response was-
"The sections will come off!
The sections will come off!
All that heat!
The soap!
The sections will come off!
I don't think I even want to try That!
I'm going to stick with Xylene and Alcohols."
Another -
" Oh, this method is /Very Unusual/.
Maybe if the graduate students try to publish a scientific paper and say that they used this method, their papers will be *Rejected* *by the Reviewers*."
"It's a published method. I have 2 published papers on it right here"
"Oh, so they can cite those methods in their papers. Ok."
*
E. Wayne Johnson
Enruikang Ag Tech
Beijing
On 9/13/2012 8:41 AM, Tony Henwood (SCHN) wrote:
> Yep
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager& Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,
> Westmead NSW 2145, AUSTRALIA
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana
> McCaig
> Sent: Wednesday, 12 September 2012 3:23 AM
> To: E. Wayne Johnson; Rene J Buesa
> Cc: histonet <@t> lists.utsouthwestern.edu; Mayer, Toysha N
> Subject: RE: [Histonet] RE: air drying special stain slides rather
> than
>
> Would this work for auto cover slipping (tape film)if they were set in the xylene reservoir prior to cover slipping?
>
> Diana
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of E.
> Wayne Johnson
> Sent: September-11-12 1:15 PM
> To: Rene J Buesa
> Cc: 'histonet <@t> lists.utsouthwestern.edu'; Mayer, Toysha N
> Subject: Re: [Histonet] RE: air drying special stain slides rather
> than
>
> I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration.
> I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me.
>
> I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven.
>
> What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok?
>
> Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us.
>
> EWJohnson
> Enruikang Ag Tech
> Beijing.
>
>
> On 9/12/2012 12:01 AM, Rene J Buesa wrote:
>
>> Toysha:
>> Perhaps you have not oven dried stained slides before, and that explains some of your comments, like:
>> 1- if the stained slides are completely dried, the "miscibility" you
>> point out is not an issues, because there is nothing to mix with;
>> 2- if you dehydrate ??? clear the stained sections that will take
>> about
>> 15 minutes per group of up to 25 slides, or even more depending on
>> the protocol used in your automated stainer, but if your group of
>> slides in their rack are placed in an oven at 60??C for 5 minutes it
>> will just that, 5 minutes reducing the usual TAT for each staining
>> procedure;
>> 3- any oven can accommodate more than 100 stained slides in their
>> racks and the TAT is shortened by oven drying, no matter how many
>> slides you are working with;
>> 4- I really do not know where you can find that "extreme heat" can
>> affect the tissue sections. All tissue sections are fixed ???
>> processed ??? dried (usually at the same 60??C before staining) ???
>> stained and an additional step at 60??C to dry before cover-slipping
>> is just that, an additional step at 60??C
>> 5- The so called "Lean" technologies do not refer to staining only,
>> they have to do with the whole work-flow and an additional drying
>> step at 60??C cannot affect in a negative way to the work-flow
>> 6- after staining you will oven dry the sections.
>> I think you should try the method instead.
>> Ren?? J.
>>
>>
>> ________________________________
>> From: "Mayer,Toysha N"<TNMayer <@t> mdanderson.org>
>> To:
>> "'histonet <@t> lists.utsouthwestern.edu'"<histonet <@t> lists.utsouthwestern.e
>> d
>> u>
>> Sent: Tuesday, September 11, 2012 11:41 AM
>> Subject: [Histonet] RE: air drying special stain slides rather than
>>
>>
>> Ooh, great question for my students next semester.
>> Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain.
>>
>> Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide.
>>
>> There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion.
>>
>>
>> Toysha N. Mayer, MBA, HT (ASCP)
>> Instructor, Education Coordinator
>> Program in Histotechnology
>> School of Health Professions
>> MD Anderson Cancer Center
>> (713) 563-3481
>> tnmayer <@t> mdanderson.org
>>
>>
>>
>>
>> Message: 16
>> Date: Tue, 11 Sep 2012 10:32:08 -0400
>> From: "Diana McCaig"<dmccaig <@t> ckha.on.ca>
>> Subject: [Histonet] air drying special stain slides rather than
>> dehydrate and clear
>> To:<histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <DCFD9E6A390E294AAF3A2561CD32E5C417A90529 <@t> ckhamail1.ckha.on.ca>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip.
>>
>>
>>
>> Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution.
>>
>>
>>
>> I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process.
>>
>>
>>
>> Diana
>>
>>
>>
>> ------------------------------
>>
>> Message: 17
>> Date: Tue, 11 Sep 2012 07:52:05 -0700 (PDT)
>> From: Rene J Buesa<rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] air drying special stain slides rather than
>> dehydrate and clear
>> To: Diana McCaig<dmccaig <@t> ckha.on.ca>,
>> "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <1347375125.72189.YahooMailNeo <@t> web121405.mail.ne1.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> Diana:
>> The most simple answer to your question is: "Because that is the way it has been done for more than 150 years".
>> The second question would be: "Is it necessary?" and the short answer to this question is: NO!!!
>> As a matter of fact, one of the steps I have developed to totally eliminate xylene from the histology lab refers to the "clearing" of stained sections, not only "special stains" (the so called HC and IHC) but the routine as well (the H&E).
>> Now, the "secret" to a successful drying of the stained slides is NOT to let them air dry because that will take not only too much time, but you can never be sure if the section is completely dry and if you add the mounting medium to a not completely dried section, you will have transparency problems.
>> The correct way of doing that is by drying the stained sections during 5 minutes at 60?C in an oven.
>> Under separate cover I am sending you something I published about your question and other aspects of how to completely eliminate xylene from ALL steps in the histology laboratory.
>> Ren? J.
>>
>>
>> ________________________________
>> From: Diana McCaig<dmccaig <@t> ckha.on.ca>
>> To: histonet <@t> lists.utsouthwestern.edu
>> Sent: Tuesday, September 11, 2012 10:32 AM
>> Subject: [Histonet] air drying special stain slides rather than
>> dehydrate and clear
>>
>> I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip.
>>
>>
>>
>> Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping.? Reason given is that the counterstain gets washed out.? Wouldn't adjusting the times be a better resolution.
>>
>>
>>
>> I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process.
>>
>>
>>
>> Diana
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>
>
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------------------------------
Message: 6
Date: Thu, 13 Sep 2012 10:49:33 -0400
From: "Hutton, Allison" <AHutton <@t> dh.org>
Subject: [Histonet] voice recognition and synoptic reporting
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<38A56C4F4630D348A50B3720409270870E0FE641 <@t> dhmail.dhorg.org>
Content-Type: text/plain; charset="iso-8859-1"
We have been asked to look at changing our pathology reports over to the synoptic report format. I was wondering if anyone could provide me some information on voice recognition software for pathology and if this would be the best (easiest) way to implement synoptic reporting for our path reports. I am only vaguely familiar with both so any information will be of great use.
Thank You in Advance
Allison
------------------------------
Message: 7
Date: Thu, 13 Sep 2012 09:04:33 -0700
From: William Chappell <chapcl <@t> yahoo.com>
Subject: Re: [Histonet] voice recognition and synoptic reporting
To: "Hutton, Allison" <AHutton <@t> dh.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <567D34AE-75D2-46C5-BDC0-8D2BA9E1EDA1 <@t> yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Voice Brook seems to be the market leader.
They are powered by Nuance and Dragon which are the market leaders in the consumer market.
However, they are pricy.
Nuance offers a service that is more similar to a remote transcription service, however my guess is they rely heavily on their automated transcription software, without the cost of purchasing the software itself.
If your IT department wants to dedicate 1 or 2 FTE's to getting you up and running, a cheaper solution is to purchase the Dragon backbone from Nuance, however it will take al ot of customization to make it on par with VoiceBrook.
Just my opinion.
Will Chappell
CHOC Children's
AP Supervisor
On Sep 13, 2012, at 7:49 AM, "Hutton, Allison" <AHutton <@t> dh.org> wrote:
> We have been asked to look at changing our pathology reports over to the synoptic report format. I was wondering if anyone could provide me some information on voice recognition software for pathology and if this would be the best (easiest) way to implement synoptic reporting for our path reports. I am only vaguely familiar with both so any information will be of great use.
> Thank You in Advance
> Allison
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 13 Sep 2012 12:41:03 -0400
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] voice recognition and synoptic reporting
To: "'Hutton, Allison'" <AHutton <@t> dh.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00e901cd91ce$919230e0$b4b692a0$@pathview.com>
Content-Type: text/plain; charset="iso-8859-1"
Allison, it starts with what your objectives are:
If you simply want your reports to present information in a certain format, then a word processor, optionally coupled with Dragon and optionally coupled with Voicebrook will work.
If you need to send the data in it's individual components to another computer system, then that's an entirely different story as it probably means that you need to store the data discretely.
Either way, if you'd like to talk about it further please feel free to contact me offline. Remember that I represent an LIS vendor, but since I love talking about this stuff, I have no problem talking to you about it from a non sales perspective.
Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Thursday, September 13, 2012 10:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] voice recognition and synoptic reporting
We have been asked to look at changing our pathology reports over to the synoptic report format. I was wondering if anyone could provide me some information on voice recognition software for pathology and if this would be the best (easiest) way to implement synoptic reporting for our path reports.
I am only vaguely familiar with both so any information will be of great use.
Thank You in Advance
Allison
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Thu, 13 Sep 2012 16:58:40 +0000
From: Cathy Crumpton <cathy.crumpton <@t> tuality.org>
Subject: [Histonet] Sakura glas coverslipper and mounting media types
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AC889E5706400B438D42F8A07AE3AF8B017F11C2 <@t> EXMBXSRV01.corp.tuality.net>
Content-Type: text/plain; charset="iso-8859-1"
For those with the Sakura Glas coverslippers, what type of mounting media are you using in these? Sakura recommends only their brand of course, but I want something that dries faster. We do not have excess mounting media on the outside of our slides, but after being in the oven for three days we had several slides sticking. It looked like the mounting media slipped down the slide to pool at the bottom and stick. I am thinking their media is too viscous. If I turn the amount down on the machine any further we end up with not enough media on the slides and get large air pockets after just a few days of storage. BTW the oven is at 40 degrees and isn't boiling hot to dry the slides.
Cathy Crumpton HT(ASCP), Lead Histotechnician
Tuality Community Hospital
503-681-1292
------------------------------
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