[Histonet] Flourescent mounting medium- PLEASE HELP!!!

[Entwistle, Laura]

We use this medium.  We have found that if you shake it before use it becomes a little more viscous, but toss it if it becomes too gel like.  We also allow our sections to dry, but keep them in the dark as much as possible.  You also need to be sure that you are using coverglass that works with fluorescent material.

Laura

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jonathan Cremer
Sent: Monday, October 08, 2012 7:20 AM
To: Candice Smoots; Histonet
Subject: RE: [Histonet] Flourescent mounting medium- PLEASE HELP!!!

I have no experience with this particular mounting medium, but from what I gather it is like any other aqueous mounting medium. You should blot your slides to remove as much water as possible, without letting your sections dry. Then apply a small amount of mounting medium to the slide or coverslip (depending on preference) and mount.
Apparently it solidifies at the edge, so no need to seal with nail polish.

If your bottle has solidified to the point where it is a gel instead of a viscous liquid, you can toss it. (unless the datasheet specifies otherwise...)

Jonathan

________________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] namens Candice Smoots [candice_camille <@t> yahoo.com]
Verzonden: maandag 8 oktober 2012 15:42
To: Histonet
Onderwerp: [Histonet] Flourescent mounting medium- PLEASE HELP!!!

Hi Histonet


In our lab, we are trying to mount our flourescent stained sections with polyvinyl alcohol mounting medium with DABCO. WE are unsure if it is to be mounted while the sections are wet or do we let me air dry.

Another question is... How to actually use it. It looks to be a little solidified. Kind of like jello. Is this normal? do I dilute it?  Please Please Help!

I remain yours truely,

Candice Camille
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