[Histonet] Polyoma for renal transplantation

[Angela K. Bitting]

We also use Calbiochem on the Ventana Ultra and XT platforms.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN)
Sent: Thursday, October 04, 2012 7:50 PM
To: 'gu.lang <@t> gmx.at'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Polyoma for renal transplantation 

Hi Gudrun,

We use the Calbiochem antibody Cat No DP02 on the Bond Platform (see below)

We originally compared it to the Novocastra's antibody and found it superior

ANTIGEN NAME:        BK Virus  
OTHER NAMES:         SV40 T Antigen
Clone:	    	 PAb416
Isotype:  	 IgG2a
		

DESCRIPTION:      Monoclonal antibody to the ~94 kDa SV40 large T antigen.
          
STORAGE CONDITIONS:        4-8oC undiluted

SUPPLIER:          Calbiochem DP02 
PROCEDURE:

Methodology:        HRP-POLYMER Working Dilution:     		Bond:	1/700 (ER2)
Special Conditions:      EDTA Heat induced epitope retrieval required 
CONTROL TISSUE:     BK Virus Control	 

Inbuilt Controls 		 
CLINICAL SIGNIFICANCE:
In immunocompromised patients, BK can cause a morphologically manifest renal infection with cytopathic signs and functional impairment. In native and transplanted kidneys, BK virus is found in areas of interstitial nephritis.
REFERENCES:
Nickeleit et al (1999) "Polyomavirus Infection of Renal Allografts" J Am Soc Nephrol 10: 1080-1089.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Friday, 5 October 2012 1:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Polyoma for renal transplantation 

Hi!

Can anyone recommend a good Polyoma antibody usefull for testing with renal transplatation.

 

Novocastra's Polyoma JC/BK is not available now.

 

Thank you

Gudrun Lang

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