[Histonet] Polyoma for renal transplantation

[Tony Henwood (SCHN)]

Hi Gudrun,

We use the Calbiochem antibody Cat No DP02 on the Bond Platform (see below)

We originally compared it to the Novocastra's antibody and found it superior

ANTIGEN NAME:        BK Virus  
OTHER NAMES:         SV40 T Antigen
Clone:	    	 PAb416
Isotype:  	 IgG2a
		

DESCRIPTION:      Monoclonal antibody to the ~94 kDa SV40 large T antigen.
          
STORAGE CONDITIONS:        4-8oC undiluted

SUPPLIER:          Calbiochem DP02 
PROCEDURE:

Methodology:        HRP-POLYMER Working Dilution:     		Bond:	1/700 (ER2)
Special Conditions:      EDTA Heat induced epitope retrieval required 
CONTROL TISSUE:     BK Virus Control	 

Inbuilt Controls 		 
CLINICAL SIGNIFICANCE:
In immunocompromised patients, BK can cause a morphologically manifest renal infection with cytopathic signs and functional impairment. In native and transplanted kidneys, BK virus is found in areas of interstitial nephritis.
REFERENCES:
Nickeleit et al (1999) "Polyomavirus Infection of Renal Allografts" J Am Soc Nephrol 10: 1080-1089.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Friday, 5 October 2012 1:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Polyoma for renal transplantation 

Hi!

Can anyone recommend a good Polyoma antibody usefull for testing with renal transplatation.

 

Novocastra's Polyoma JC/BK is not available now.

 

Thank you

Gudrun Lang

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