AW: [Histonet] Polyoma for renal transplantation

Gudrun Lang gu.lang <@t>
Fri Oct 5 10:15:56 CDT 2012

Many thanks for all the kind responses regarding the Polyoma antibody.
: )


-----Ursprüngliche Nachricht-----
Von: Angela K. Bitting [mailto:akbitting <@t>] 
Gesendet: Freitag, 05. Oktober 2012 14:40
An: Tony Henwood (SCHN); 'gu.lang <@t>'; histonet <@t>
Betreff: RE: [Histonet] Polyoma for renal transplantation 

We also use Calbiochem on the Ventana Ultra and XT platforms.

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Tony Henwood
Sent: Thursday, October 04, 2012 7:50 PM
To: 'gu.lang <@t>'; histonet <@t>
Subject: RE: [Histonet] Polyoma for renal transplantation 

Hi Gudrun,

We use the Calbiochem antibody Cat No DP02 on the Bond Platform (see below)

We originally compared it to the Novocastra's antibody and found it superior

ANTIGEN NAME:        BK Virus  
OTHER NAMES:         SV40 T Antigen
Clone:	    	 PAb416
Isotype:  	 IgG2a

DESCRIPTION:      Monoclonal antibody to the ~94 kDa SV40 large T antigen.
STORAGE CONDITIONS:        4-8oC undiluted

SUPPLIER:          Calbiochem DP02 

Methodology:        HRP-POLYMER Working Dilution:     		Bond:
1/700 (ER2)
Special Conditions:      EDTA Heat induced epitope retrieval required 
CONTROL TISSUE:     BK Virus Control	 

Inbuilt Controls 		 
In immunocompromised patients, BK can cause a morphologically manifest renal
infection with cytopathic signs and functional impairment. In native and
transplanted kidneys, BK virus is found in areas of interstitial nephritis.
Nickeleit et al (1999) "Polyomavirus Infection of Renal Allografts" J Am Soc
Nephrol 10: 1080-1089.

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,
Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Gudrun Lang
Sent: Friday, 5 October 2012 1:37 AM
To: histonet <@t>
Subject: [Histonet] Polyoma for renal transplantation 


Can anyone recommend a good Polyoma antibody usefull for testing with renal


Novocastra's Polyoma JC/BK is not available now.


Thank you

Gudrun Lang

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