[Histonet] Polyoma for renal transplantation

Lauren Hopson lhopson <@t> cellmarque.com
Thu Oct 4 13:34:08 CDT 2012

Hello Gudrun,

The SV40 antibody from Cell Marque is intended for use on FFPE tissue and is compatible with multiple staining platforms. 

Here is some information that I found regarding Simian Virus 40:

Polyomavirus BK (BKV) has been classified in the Polyomaviridae family, which is subdivided into several genetically distinct groups. One of these groups, the simian virus 40 (SV40) subgroup, contains the four known human polyomaviruses: BK, JC, KI, and WU. BKV-associated nephropathy affecting a kidney transplant was first described as a single case report by a pathologist Mackenziein in 1978, and the virus was named after the patient, whose initials were B.K. In the following years after the initial case report, BKV was largely ''forgotten,'' and a significant incidence of polyomavirus infection in the renal transplant population has been seen only in recent years. BK and JC virus are similar in that their genome sequences share 75% homology, and both viruses tend to be problematic primarily in the immunocompromised population. JC virus is the etiologic agent of progressive multifocal leukoencephalopathy (PML), which is mainly seen in HIV patients, organ transplant recipients, and other immunodeficient syndromes. Although both JC and BK virus have been implicated in some cases of polyomavirus-associated nephropathy, the vast majority of cases are caused by BK virus. The cytopathic effects of BKV can be difficult to distinguish from those caused by other viral infections, such as adenovirus. Immunohistochemical staining for simian virus 40 (SV40) is often necessary to distinguish BKV from other viruses with similar cytopathic effects. SV40 is a member of the Polyomaviridae family that is found in both monkeys and humans, and immunostaining for SV40 highlights all polyoma viral infections because of cross-reactivity between SV40 and both BKV and JC virus.

Additional information can be found on our website. 

Best Regards,

Lauren Hopson
International Product Specialist

6600 Sierra College Blvd.
Rocklin, CA 95677
(916) 746-8900 x8929
(916) 996-2353 (mobile)
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Thursday, October 04, 2012 11:17 AM
To: 'Richard Cartun'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] Polyoma for renal transplantation

Our protocol on Ventana Benchmark Ultra: CC1 mild (ca. 30 min high pH retrieval), 1:40 for 32 min at 37°C.

We use this special lot since 2006, now aliquotted and frozen. I am sure (or hope), that we've got a positiv result, when introducing it. 
Unfortunately we have no positive controls available. So I'm not convinced of the reliability today. :( It will be challenging to establish the new antibody.

I found a SV40 antibody from Cellmarque, but I'm not sure, if this one is the relevant one for renal transplants. Publications always talk about BK-Virus.


-----Ursprüngliche Nachricht-----
Von: Richard Cartun [mailto:Rcartun <@t> harthosp.org]
Gesendet: Donnerstag, 04. Oktober 2012 18:52
An: gu.lang <@t> gmx.at
Betreff: Re: [Histonet] Polyoma for renal transplantation

I forgot to ask if you were able to get the Novocastra antibody to work on FFPE tissue?  Thank you.


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-2204 Fax

>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 10/4/2012 11:36 AM >>>

Can anyone recommend a good Polyoma antibody usefull for testing with renal transplatation.


Novocastra's Polyoma JC/BK is not available now.


Thank you

Gudrun Lang

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