[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!

[Edwards, Richard E.]

Perhaps all is not lost  as  you  will be  able  to  see  on a  H <@t> E the  spaces reluctantly vacated by  the  lipids.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of z o n k e d
Sent: 13 November 2012 17:44
To: Jennifer MacDonald
Cc: histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!

We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened.

On Tuesday, November 13, 2012, Jennifer MacDonald wrote:

> It depends on what you are using the oil red o for.  Lipofuscin and 
> ceroid can be demonstrated with an oil red o stain after processing.
> Jennifer MacDonald
>
>
>
>
> From:        z o n k e d <zonked <@t> gmail.com <javascript:_e({}, 'cvml',
> 'zonked <@t> gmail.com');>>
> To:        "histonet <@t> lists.utsouthwestern.edu <javascript:_e({}, 'cvml',
> 'histonet <@t> lists.utsouthwestern.edu');>" 
> <histonet <@t> lists.utsouthwestern.edu<javascript:_e({}, 'cvml', 
> 'histonet <@t> lists.utsouthwestern.edu');>
> >
> Date:        11/13/2012 08:53 AM
> Subject:        [Histonet] Help! Liver mistakenly processed in paraffin
> (had to be        in OCT instead)!
> Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu<javascript:_e({}, 'cvml', 'histonet-bounces <@t> lists.utsouthwestern.edu');>
> ------------------------------
>
>
>
> Hello Histonetters,
>
> First time writer, long time reader. I'm a newbie tech in academia and 
> I was given a simple task which I think I pretty much screwed up.
>
> I should have embedded half of a mouse liver in paraffin for microtome 
> sectioning while the other half should have been embedded in OCT for 
> cryosectioning (for oil red o). I made the mistake last night of 
> placing both liver halves into the tissue processor. The liver I 
> intended for OCT embedding is now hard as wax. Is there any way to 
> deparaffinize processed organs and may I embed them in OCT for proper 
> cryosectioning? I imagine that the liver would get dehydrated, I would 
> get crappy sections, and Oil Red O won't work.
>
> Any suggestions are welcome.
>
> Thank you so much,
>
> Zoe W.
>
>
> --
> "It costs nothing to say something kind. Even less to shut up altogether."
>
>    --Nathan Fillion
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--
"It costs nothing to say something kind. Even less to shut up altogether."

    --Nathan Fillion
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