[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!

[Jennifer MacDonald]

if there was fat replacement, such as cirrhosis, you will see it in the 
morphology.



From:   z o n k e d <zonked <@t> gmail.com>
To:     Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Cc:     "histonet <@t> lists.utsouthwestern.edu" 
<histonet <@t> lists.utsouthwestern.edu>, 
"histonet-bounces <@t> lists.utsouthwestern.edu" 
<histonet-bounces <@t> lists.utsouthwestern.edu>
Date:   11/13/2012 09:43 AM
Subject:        Re: Help! Liver mistakenly processed in paraffin (had to 
be in OCT instead)!



We just wanted to see general lipids, nothing in particular. This mouse 
died unexpectedly and may have been part of a group that was put on a high 
fat or high bile acid diet and we just wanted to see what happened.

On Tuesday, November 13, 2012, Jennifer MacDonald wrote:
It depends on what you are using the oil red o for.  Lipofuscin and ceroid 
can be demonstrated with an oil red o stain after processing. 
Jennifer MacDonald 




From:        z o n k e d <zonked <@t> gmail.com> 
To:        "histonet <@t> lists.utsouthwestern.edu" <
histonet <@t> lists.utsouthwestern.edu> 
Date:        11/13/2012 08:53 AM 
Subject:        [Histonet] Help! Liver mistakenly processed in paraffin 
(had to be        in OCT instead)! 
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu 



Hello Histonetters,

First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.

I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of placing
both liver halves into the tissue processor. The liver I intended for OCT
embedding is now hard as wax. Is there any way to deparaffinize processed
organs and may I embed them in OCT for proper cryosectioning? I imagine
that the liver would get dehydrated, I would get crappy sections, and Oil
Red O won't work.

Any suggestions are welcome.

Thank you so much,

Zoe W.


-- 
"It costs nothing to say something kind. Even less to shut up altogether."

   --Nathan Fillion
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-- 
"It costs nothing to say something kind. Even less to shut up altogether."

    --Nathan Fillion