[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!

[z o n k e d]

We just wanted to see general lipids, nothing in particular. This mouse
died unexpectedly and may have been part of a group that was put on a high
fat or high bile acid diet and we just wanted to see what happened.

On Tuesday, November 13, 2012, Jennifer MacDonald wrote:

> It depends on what you are using the oil red o for.  Lipofuscin and ceroid
> can be demonstrated with an oil red o stain after processing.
> Jennifer MacDonald
>
>
>
>
> From:        z o n k e d <zonked <@t> gmail.com <javascript:_e({}, 'cvml',
> 'zonked <@t> gmail.com');>>
> To:        "histonet <@t> lists.utsouthwestern.edu <javascript:_e({}, 'cvml',
> 'histonet <@t> lists.utsouthwestern.edu');>" <histonet <@t> lists.utsouthwestern.edu<javascript:_e({}, 'cvml', 'histonet <@t> lists.utsouthwestern.edu');>
> >
> Date:        11/13/2012 08:53 AM
> Subject:        [Histonet] Help! Liver mistakenly processed in paraffin
> (had to be        in OCT instead)!
> Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu<javascript:_e({}, 'cvml', 'histonet-bounces <@t> lists.utsouthwestern.edu');>
> ------------------------------
>
>
>
> Hello Histonetters,
>
> First time writer, long time reader. I'm a newbie tech in academia and I
> was given a simple task which I think I pretty much screwed up.
>
> I should have embedded half of a mouse liver in paraffin for microtome
> sectioning while the other half should have been embedded in OCT for
> cryosectioning (for oil red o). I made the mistake last night of placing
> both liver halves into the tissue processor. The liver I intended for OCT
> embedding is now hard as wax. Is there any way to deparaffinize processed
> organs and may I embed them in OCT for proper cryosectioning? I imagine
> that the liver would get dehydrated, I would get crappy sections, and Oil
> Red O won't work.
>
> Any suggestions are welcome.
>
> Thank you so much,
>
> Zoe W.
>
>
> --
> "It costs nothing to say something kind. Even less to shut up altogether."
>
>    --Nathan Fillion
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-- 
"It costs nothing to say something kind. Even less to shut up altogether."

    --Nathan Fillion