[Histonet] Help! Liver mistakenly processed in paraffin (had to
be in OCT instead)!
JMacDonald <@t> mtsac.edu
Tue Nov 13 11:14:31 CST 2012
It depends on what you are using the oil red o for. Lipofuscin and ceroid
can be demonstrated with an oil red o stain after processing.
From: z o n k e d <zonked <@t> gmail.com>
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Date: 11/13/2012 08:53 AM
Subject: [Histonet] Help! Liver mistakenly processed in paraffin
(had to be in OCT instead)!
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of placing
both liver halves into the tissue processor. The liver I intended for OCT
embedding is now hard as wax. Is there any way to deparaffinize processed
organs and may I embed them in OCT for proper cryosectioning? I imagine
that the liver would get dehydrated, I would get crappy sections, and Oil
Red O won't work.
Any suggestions are welcome.
Thank you so much,
"It costs nothing to say something kind. Even less to shut up altogether."
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