[Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!

Rena Fail renafail2 <@t> gmail.com
Tue Nov 13 11:10:16 CST 2012

Oil Red O is a stain for fat, Alcohol dissolves fat Rehydrating  won't
help. You  can't replace the fat.
Rena Fail

On Tue, Nov 13, 2012 at 11:52 AM, z o n k e d <zonked <@t> gmail.com> wrote:

> Hello Histonetters,
> First time writer, long time reader. I'm a newbie tech in academia and I
> was given a simple task which I think I pretty much screwed up.
> I should have embedded half of a mouse liver in paraffin for microtome
> sectioning while the other half should have been embedded in OCT for
> cryosectioning (for oil red o). I made the mistake last night of placing
> both liver halves into the tissue processor. The liver I intended for OCT
> embedding is now hard as wax. Is there any way to deparaffinize processed
> organs and may I embed them in OCT for proper cryosectioning? I imagine
> that the liver would get dehydrated, I would get crappy sections, and Oil
> Red O won't work.
> Any suggestions are welcome.
> Thank you so much,
> Zoe W.
> --
> "It costs nothing to say something kind. Even less to shut up altogether."
>     --Nathan Fillion
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