[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to
be in OCT instead)!
z o n k e d
zonked <@t> gmail.com
Tue Nov 13 11:11:16 CST 2012
Ah, I knew it. :(
On Tuesday, November 13, 2012, Will Chappell wrote:
> Nope, sorry. All your fat is dissolved.
> Sent from my iPhone
> > Hello Histonetters,
> > First time writer, long time reader. I'm a newbie tech in academia and I
> > was given a simple task which I think I pretty much screwed up.
> > I should have embedded half of a mouse liver in paraffin for microtome
> > sectioning while the other half should have been embedded in OCT for
> > cryosectioning (for oil red o). I made the mistake last night of placing
> > both liver halves into the tissue processor. The liver I intended for OCT
> > embedding is now hard as wax. Is there any way to deparaffinize processed
> > organs and may I embed them in OCT for proper cryosectioning? I imagine
> > that the liver would get dehydrated, I would get crappy sections, and Oil
> > Red O won't work.
> > Any suggestions are welcome.
> > Thank you so much,
> > Zoe W.
> > --
> > "It costs nothing to say something kind. Even less to shut up
> > --Nathan Fillion
> > _______________________________________________
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> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
"It costs nothing to say something kind. Even less to shut up altogether."
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