[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!

[z o n k e d]

Ah, I knew it. :(
Thank you.

Zoe

On Tuesday, November 13, 2012, Will Chappell wrote:

> Nope, sorry. All your fat is dissolved.
>
> Sent from my iPhone
>
> On Nov 13, 2012, at 8:52 AM, z o n k e d <zonked <@t> gmail.com <javascript:;>>
> wrote:
>
> > Hello Histonetters,
> >
> > First time writer, long time reader. I'm a newbie tech in academia and I
> > was given a simple task which I think I pretty much screwed up.
> >
> > I should have embedded half of a mouse liver in paraffin for microtome
> > sectioning while the other half should have been embedded in OCT for
> > cryosectioning (for oil red o). I made the mistake last night of placing
> > both liver halves into the tissue processor. The liver I intended for OCT
> > embedding is now hard as wax. Is there any way to deparaffinize processed
> > organs and may I embed them in OCT for proper cryosectioning? I imagine
> > that the liver would get dehydrated, I would get crappy sections, and Oil
> > Red O won't work.
> >
> > Any suggestions are welcome.
> >
> > Thank you so much,
> >
> > Zoe W.
> >
> >
> > --
> > "It costs nothing to say something kind. Even less to shut up
> altogether."
> >
> >    --Nathan Fillion
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu <javascript:;>
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
"It costs nothing to say something kind. Even less to shut up altogether."

    --Nathan Fillion