[Histonet] Help! Liver mistakenly processed in paraffin (had to
be in OCT instead)!
chapcl <@t> yahoo.com
Tue Nov 13 11:00:24 CST 2012
Nope, sorry. All your fat is dissolved.
Sent from my iPhone
On Nov 13, 2012, at 8:52 AM, z o n k e d <zonked <@t> gmail.com> wrote:
> Hello Histonetters,
> First time writer, long time reader. I'm a newbie tech in academia and I
> was given a simple task which I think I pretty much screwed up.
> I should have embedded half of a mouse liver in paraffin for microtome
> sectioning while the other half should have been embedded in OCT for
> cryosectioning (for oil red o). I made the mistake last night of placing
> both liver halves into the tissue processor. The liver I intended for OCT
> embedding is now hard as wax. Is there any way to deparaffinize processed
> organs and may I embed them in OCT for proper cryosectioning? I imagine
> that the liver would get dehydrated, I would get crappy sections, and Oil
> Red O won't work.
> Any suggestions are welcome.
> Thank you so much,
> Zoe W.
> "It costs nothing to say something kind. Even less to shut up altogether."
> --Nathan Fillion
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