[Histonet] Re: Histonet Digest, Vol 102, Issue 28

[Amos Brooks]

Hi,
   I have done this. It works quite well. It would probably be best to use
an alkaline phosphatase detection since the brilliant red of the fast red
precipitate is more distinguishable from the brown DAB. (I am assuming you
used HRP-DAB detection of the Tunel.) You needn't worry about the left over
HRP since you'll be doing an antigen retrieval step which should wash them
all out.

Amos

On Wed, May 23, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 16
> Date: Wed, 23 May 2012 08:03:17 -0700
> From: "Alicia R. Lange" <arlange <@t> medicine.nevada.edu>
> Subject: [Histonet] RESTAINING WITH KI67
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <9809822F80B1414C90EB4438713C896801345590 <@t> MEDX.medicine.nevada.edu>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hello Histoland!
>
>
>
> Has anyone restained tunel slides with ki67? Since tunel stains
> apoptosis and ki67 proliferation, I was just going to restain with ki67.
> I wanted to get some input before I did this. Thanks!
>
>
>
> Alicia Lange
>