[Histonet] RNA extraction and amplification of LCM rice grain tissue

[Marlen Müller]

Dear HistoNet Community,

Currently we are working on a procedure to dissect the grain of the rice 
plant via a PALM Laser Microdisection System. According to published 
work in the barley grain (Schiebold et al 2011) 2 kits for RNA 
extraction, purification and amplification were used (for mRNA 
extraction "Dynabeads mRNA DIRECT MicroKit" or for total RNA 
extraction"RNeasy MicroKit" and for RNA amplification "C&E Version 
ExpressArt mRNA amplification Nanokit"). We will use the extracted and 
amplified RNA then for high-throughput RNA Sequencing.
Do you have any experience with RNA amplification for very small amount 
of tissues? Would you use the classical trizol method or should I rely 
on a commercial kit for extraction?
Thanks alot for sharing your knowledge!

Best regards,
Marlen

-- 
ETH Zürich
*Marlen Müller*
PhD Student
Group of Plantbiotechnology
Universitätstrasse 2
8092 Zürich
Switzerland