AW: [Histonet] Immunofluorescence in the clinical laboratory, some questions

[Gudrun Lang]

Hi Gayle!
We do mainly the same as Patrick. Dermatological specimens for bull.
deseases on frozen air-dried slides. Direct IF + indirect IF with patients
serum.
We do also direct IF on NBF fixed renal biopsies, with digestion for antigen
retrieval.
We perform those tests manually.
2-3 skinbiopsies per week, 3-4 renal biopsies per month. Only clinical.

Best regards
Gudrun


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von gayle
callis
Gesendet: Samstag, 12. Mai 2012 16:48
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Immunofluorescence in the clinical laboratory, some
questions

Dear Histonetters, 

 

I know that immunofluorescence has been done for decades on renal biopsies,
but am curious if laboratories are using IF more these days?   If so, I
would be very interested to talk to you one on one about this as I have more
questions on why you deviate from standard chromogenic enzyme
immunohistochemistry and perform IF.   Comments about renal biopsy
procedures are welcome too.  

 

  Also, do you do mostly single IF or double IF, and the reasons why?   Is
your IF done primarily on FFPE or frozen sections/acetone fixation?  

 

When you do immunofluorescence in your clinical laboratory are you using an
automated stainer or a manual protocol?   Or is your clinical laboratory
associated with medical research groups?   

 

Any comments/information is most welcome.  

 

Thanks..............

 

Gayle M. Callis

HTL/HT/MT(ASCP)  

Bozeman MT

 

 

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