[Histonet] Re: Histonet Digest, Vol 102, Issue 13
Rory Pritchard
rory.pritchard001 <@t> gmail.com
Sat May 12 12:04:38 CDT 2012
Message 3: xylene substitute for counterstain clearing.
You could use histoclear, it's less toxic than xylene and works well.
-Rory
On May 12, 2012, at 1:00 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Fwd: Plants (Behnaz Sohrab)
> 2. Re: Fwd: Plants (William)
> 3. RE: Xylene Substitute for Counterstain Clearing (Cynthia Pyse)
> 4. paraffin melting in VIP (Gudrun Lang)
> 5. Re: Fwd: Plants (Victoria Baker)
> 6. Re: Xylene Substitute for Counterstain Clearing (Victoria Baker)
> 7. Re: Xylene Substitute for Counterstain Clearing (Rene J Buesa)
> 8. Re: paraffin melting in VIP (Rene J Buesa)
> 9. Re: paraffin melting in VIP (Jennifer Campbell)
> 10. RE: paraffin melting in VIP (Sarah Dysart)
> 11. AW: [Histonet] paraffin melting in VIP (Gudrun Lang)
> 12. RE: paraffin melting in VIP (Goins, Tresa)
> 13. Re: Fwd: Plants (Kim Donadio)
> 14. Re: Fwd: Plants (Lucie Guernsey)
> 15. RE: Xylene Substitute for Counterstain Clearing (gayle callis)
> 16. RE: Fwd: Plants (Sebree Linda A)
> 17. RE: Fwd: Plants (Sebree Linda A)
> 18. Re: Fwd: Plants (Emily Sours)
> 19. Immunofluorescence in the clinical laboratory, some questions
> (gayle callis)
> 20. Re: Immunofluorescence in the clinical laboratory, some
> questions (Patrick Laurie)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 11 May 2012 10:29:59 -0700
> From: "Behnaz Sohrab" <SohrabB1 <@t> ah.org>
> Subject: [Histonet] Fwd: Plants
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4FACEA27.4347.0054.1 <@t> ah.org>
> Content-Type: text/plain; charset="us-ascii"
>
>
> I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this?
> Thank you, Behnaz
>
> ------------------------------
>
> Message: 2
> Date: Fri, 11 May 2012 13:32:37 -0400
> From: William <chapcl <@t> yahoo.com>
> Subject: Re: [Histonet] Fwd: Plants
> To: Behnaz Sohrab <SohrabB1 <@t> ah.org>
> Cc: "<histonet <@t> lists.utsouthwestern.edu>"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <0EC9573D-D1AD-40B2-B9A0-E0CB4991E2BA <@t> yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> I have had plants in a number of labs. Could be against the rules, but I never saw it. I even had a canary in one lab - pretty sure that is against the rules.
>
> Will Chappell
>
> Sent from my iPhone
>
> On May 11, 2012, at 1:29 PM, "Behnaz Sohrab" <SohrabB1 <@t> ah.org> wrote:
>
>>
>> I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this?
>> Thank you, Behnaz
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 11 May 2012 13:41:33 -0400
> From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
> Subject: RE: [Histonet] Xylene Substitute for Counterstain Clearing
> To: "'Andrew Coleman'" <andrewcoleman131 <@t> gmail.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001e01cd2f9d$4eaeec70$ec0cc550$@com>
> Content-Type: text/plain; charset="us-ascii"
>
> Andrew
> You could use Clearium from Leica. Clearium can either be coverslipped from
> xylene or isopropyl alcohol. Drying time from isopropyl is longer then
> xylene.
> Cindy
>
> Cindy Pyse, CLT, HT (ASCP)
> Laboratory Manager
> X-Cell Laboratories
> 716-250-9235 etx. 232
> e-mail cpyse <@t> x-celllab.com
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrew
> Coleman
> Sent: Friday, May 11, 2012 12:25 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Xylene Substitute for Counterstain Clearing
>
> Hi all,
>
> We are performing a neutral red counterstain on tissue sections containing
> colored polystyrene microspheres. The spheres are inert to alcohol, but are
> washed out when we clear with xylene to coverslip.
> The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
> chloroform and methylene chloride for what its worth.
>
> Is it reasonable to coverslip these slides in permanent mount without
> clearing with xylene after dehydrating the tissue? Or does anyone know of a
> substitute clearing agent with chemical properties dissimilar enough from
> xylene that might be worth trying instead?
>
> Thanks,
>
> Andrew
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 11 May 2012 19:58:39 +0200
> From: "Gudrun Lang" <gu.lang <@t> gmx.at>
> Subject: [Histonet] paraffin melting in VIP
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001b01cd2f9f$b24a4610$16ded230$@gmx.at>
> Content-Type: text/plain; charset="us-ascii"
>
> HI!
>
> A question for those, who melt the paraffin directly in the VIP. How long
> does it take to melt the pellets in the VIP-oven?
>
>
>
> Thanks
>
> Gudrun
>
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 11 May 2012 14:17:39 -0400
> From: Victoria Baker <bakevictoria <@t> gmail.com>
> Subject: Re: [Histonet] Fwd: Plants
> To: Behnaz Sohrab <SohrabB1 <@t> ah.org>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CALYZRmS65GqSk46JbCPaPvbG=6YZwuR1_mS94oM9T18R4MX_Sg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> It's probably more toxic for the plants, but I like having them and no one
> has told me I had to remove them. Ivy's are the most sturdy and the green
> color just perks up things.
> On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab <SohrabB1 <@t> ah.org> wrote:
>
>>
>> I was told by infectious control person that plants are not allowed in the
>> lab?? IS this true? any experience with this?
>> Thank you, Behnaz
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 11 May 2012 14:21:45 -0400
> From: Victoria Baker <bakevictoria <@t> gmail.com>
> Subject: Re: [Histonet] Xylene Substitute for Counterstain Clearing
> To: Andrew Coleman <andrewcoleman131 <@t> gmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CALYZRmQ=dPi8CvNVXPMXo=zaQFd8BAzV6KHCJVz16wqe2ySGrA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> I think that it is called Crystal Mount - but apply to section, allow to
> harden dip slide in clearing media and coverslip. I know that there must
> be others out there as well.
> Good Luck
>
>
>
> On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman <andrewcoleman131 <@t> gmail.com
>> wrote:
>
>> Hi all,
>>
>> We are performing a neutral red counterstain on tissue sections
>> containing colored polystyrene microspheres. The spheres are inert to
>> alcohol, but are washed out when we clear with xylene to coverslip.
>> The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
>> chloroform and methylene chloride for what its worth.
>>
>> Is it reasonable to coverslip these slides in permanent mount without
>> clearing with xylene after dehydrating the tissue? Or does anyone know
>> of a substitute clearing agent with chemical properties dissimilar
>> enough from xylene that might be worth trying instead?
>>
>> Thanks,
>>
>> Andrew
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 11 May 2012 11:22:57 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Xylene Substitute for Counterstain Clearing
> To: histonet <@t> lists.utsouthwestern.edu, Andrew Coleman
> <andrewcoleman131 <@t> gmail.com>
> Message-ID:
> <1336760577.37265.YahooMailClassic <@t> web162101.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> After staining dry the sections in an oven at 60�C for 10 minutes. When completely dried, coverslip as usual. Beware of the mounting medium solvent because it may contain xylene as well. Use one mounting medium without xylene.
> Ren� J.
>
> --- On Fri, 5/11/12, Andrew Coleman <andrewcoleman131 <@t> gmail.com> wrote:
>
>
> From: Andrew Coleman <andrewcoleman131 <@t> gmail.com>
> Subject: [Histonet] Xylene Substitute for Counterstain Clearing
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, May 11, 2012, 12:25 PM
>
>
> Hi all,
>
> We are performing a neutral red counterstain on tissue sections
> containing colored polystyrene microspheres. The spheres are inert to
> alcohol, but are washed out when we clear with xylene to coverslip.
> The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
> chloroform and methylene chloride for what its worth.
>
> Is it reasonable to coverslip these slides in permanent mount without
> clearing with xylene after dehydrating the tissue? Or does anyone know
> of a substitute clearing agent with chemical properties dissimilar
> enough from xylene that might be worth trying instead?
>
> Thanks,
>
> Andrew
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 11 May 2012 11:27:36 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] paraffin melting in VIP
> To: histonet <@t> lists.utsouthwestern.edu, gu.lang <@t> gmx.at
> Message-ID:
> <1336760856.98785.YahooMailClassic <@t> web162102.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Regardless of the time it takes or of how many people do it, melting the paraffin directly in the VIP should not be done because it causes the heating elements to work extra reducing their useful life. They are quite expensive to replace!.
> Melt the paraffin outside the VIP and use the VIP only to keep the melted paraffin�at the temperature you desire.
> Ren� J.
>
> --- On Fri, 5/11/12, Gudrun Lang <gu.lang <@t> gmx.at> wrote:
>
>
> From: Gudrun Lang <gu.lang <@t> gmx.at>
> Subject: [Histonet] paraffin melting in VIP
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, May 11, 2012, 1:58 PM
>
>
> HI!
>
> A question for those, who melt the paraffin directly in the VIP. How long
> does it take to melt the pellets in the VIP-oven?
>
>
>
> Thanks
>
> Gudrun
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 9
> Date: Fri, 11 May 2012 14:37:21 -0400
> From: Jennifer Campbell <campbellj <@t> muhlbauerlab.com>
> Subject: Re: [Histonet] paraffin melting in VIP
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAEK9oWiBRVb5OWUeuZ32sPmwWwkaZQXdb0NhRqbD075MGp_b5g <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> I agree Rene!
>
> I also believe Sakura recommends not putting paraffin flakes directly in
> the containers.
>
> On Fri, May 11, 2012 at 2:27 PM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>
>> Regardless of the time it takes or of how many people do it, melting the
>> paraffin directly in the VIP should not be done because it causes the
>> heating elements to work extra reducing their useful life. They are quite
>> expensive to replace!.
>> Melt the paraffin outside the VIP and use the VIP only to keep the melted
>> paraffin at the temperature you desire.
>> Ren� J.
>>
>> --- On Fri, 5/11/12, Gudrun Lang <gu.lang <@t> gmx.at> wrote:
>>
>>
>> From: Gudrun Lang <gu.lang <@t> gmx.at>
>> Subject: [Histonet] paraffin melting in VIP
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Friday, May 11, 2012, 1:58 PM
>>
>>
>> HI!
>>
>> A question for those, who melt the paraffin directly in the VIP. How long
>> does it take to melt the pellets in the VIP-oven?
>>
>>
>>
>> Thanks
>>
>> Gudrun
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Jen Campbell, HT(ASCP)
> Supervisor of Technical Services
> Muhlbauer Dermatopathology Laboratory
> 61 Monroe Avenue, Ste B
> Pittsford NY 14534
> P: 585.586.5166
> F: 585.586.3137
>
>
> IMPORTANT NOTICE: This e-mail and any attachments may contain confidential
> or sensitive information which is, or may be, legally privileged or
> otherwise protected by law from further disclosure. It is intended only
> for the addressee. If you received this in error or from someone who was
> not authorized to send it to you, please do not distribute, copy or use it
> or any attachments. Please notify the sender immediately by reply e-mail
> and delete this from your system. Thank you for your cooperation.
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 11 May 2012 18:51:56 +0000
> From: Sarah Dysart <sdysart <@t> mirnarx.com>
> Subject: RE: [Histonet] paraffin melting in VIP
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, "gu.lang <@t> gmx.at" <gu.lang <@t> gmx.at>
> Message-ID:
> <8A70A9B2ECDD084DACFE6C59FCF86D50066E2135 <@t> BL2PRD0710MB363.namprd07.prod.outlook.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> This is probably a best case scenario, but for labs like the one I am currently in I melt it in the processor. My lab doesn't have the funds to buy me a melting pot (I have had them in all my other labs, just don't know what they are technically called).
> To answer your question it usually takes overnight and it's melted, but sometimes I end up having to add a little more to get to top off level, that takes a couple hours.
> Good Luck!!
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Friday, May 11, 2012 1:28 PM
> To: histonet <@t> lists.utsouthwestern.edu; gu.lang <@t> gmx.at
> Subject: Re: [Histonet] paraffin melting in VIP
>
> Regardless of the time it takes or of how many people do it, melting the paraffin directly in the VIP should not be done because it causes the heating elements to work extra reducing their useful life. They are quite expensive to replace!.
> Melt the paraffin outside the VIP and use the VIP only to keep the melted paraffin�at the temperature you desire.
> Ren� J.
>
> --- On Fri, 5/11/12, Gudrun Lang <gu.lang <@t> gmx.at> wrote:
>
>
> From: Gudrun Lang <gu.lang <@t> gmx.at>
> Subject: [Histonet] paraffin melting in VIP
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, May 11, 2012, 1:58 PM
>
>
> HI!
>
> A question for those, who melt the paraffin directly in the VIP. How long
> does it take to melt the pellets in the VIP-oven?
>
>
>
> Thanks
>
> Gudrun
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Fri, 11 May 2012 21:31:00 +0200
> From: "Gudrun Lang" <gu.lang <@t> gmx.at>
> Subject: AW: [Histonet] paraffin melting in VIP
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <003b01cd2fac$98dca5d0$ca95f170$@gmx.at>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Thanks for your kind responses!
> Gudrun
>
> -----Urspr�ngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun
> Lang
> Gesendet: Freitag, 11. Mai 2012 19:59
> An: histon
More information about the Histonet
mailing list