[Histonet] Immunofluorescence in the clinical laboratory, some questions

[Patrick Laurie]

Hi Gayle,

We perform IF on renal biopsies and dermatological specimens with various
bullous disorders.  We perform IgA, IgM, IgG, C3, C1q, Albumin, Fibrinogen,
Kappa and Lambda on acetone fixed frozen sections.  These are all direct
IF.  We perform C4D indirectly on frozen tissue due to the lack of staining
intensity of any directly conjugated antibodies, as well as C4D IF on FFPE
tissue and we also perform IHC C4D on FFPE.  Our pathologist's usual
comments about the reason that they like the immunofluorescence is that
they can easily see smaller deposits using immunofluorescence that would be
indeterminate with conventional IHC.

We use an automated stainer for these, it allows us to be more hands off as
well as to incubate them in the dark.

Let me know if you have any further questions.

On Sat, May 12, 2012 at 7:48 AM, gayle callis <gayle.callis <@t> bresnan.net>wrote:

> Dear Histonetters,
>
>
>
> I know that immunofluorescence has been done for decades on renal biopsies,
> but am curious if laboratories are using IF more these days?   If so, I
> would be very interested to talk to you one on one about this as I have
> more
> questions on why you deviate from standard chromogenic enzyme
> immunohistochemistry and perform IF.   Comments about renal biopsy
> procedures are welcome too.
>
>
>
>  Also, do you do mostly single IF or double IF, and the reasons why?   Is
> your IF done primarily on FFPE or frozen sections/acetone fixation?
>
>
>
> When you do immunofluorescence in your clinical laboratory are you using an
> automated stainer or a manual protocol?   Or is your clinical laboratory
> associated with medical research groups?
>
>
>
> Any comments/information is most welcome.
>
>
>
> Thanks..............
>
>
>
> Gayle M. Callis
>
> HTL/HT/MT(ASCP)
>
> Bozeman MT
>
>
>
>
>
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com