[Histonet] Xylene Substitute for Counterstain Clearing

[Cynthia Pyse]

Andrew
You could use Clearium from Leica. Clearium can either be coverslipped from
xylene or isopropyl alcohol. Drying time from isopropyl is longer then
xylene.
Cindy

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cpyse <@t> x-celllab.com



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrew
Coleman
Sent: Friday, May 11, 2012 12:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute for Counterstain Clearing

Hi all,

We are performing a neutral red counterstain on tissue sections containing
colored polystyrene microspheres. The spheres are inert to alcohol, but are
washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and methylene chloride for what its worth.

Is it reasonable to coverslip these slides in permanent mount without
clearing with xylene after dehydrating the tissue? Or does anyone know of a
substitute clearing agent with chemical properties dissimilar enough from
xylene that might be worth trying instead?

Thanks,

Andrew

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