[Histonet] Xylene Substitute for Counterstain Clearing

[Elizabeth Chlipala]

Andrew

There are aqueous permanent mounting medias such as Advantage Permanent Mounting Media from Accurate Chemical NB300A (516) 333-2221 its been years since I used it but it does work on some applications.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrew Coleman
Sent: Friday, May 11, 2012 10:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute for Counterstain Clearing

Hi all,

We are performing a neutral red counterstain on tissue sections
containing colored polystyrene microspheres. The spheres are inert to
alcohol, but are washed out when we clear with xylene to coverslip.
The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
chloroform and methylene chloride for what its worth.

Is it reasonable to coverslip these slides in permanent mount without
clearing with xylene after dehydrating the tissue? Or does anyone know
of a substitute clearing agent with chemical properties dissimilar
enough from xylene that might be worth trying instead?

Thanks,

Andrew

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