[Histonet] Xylene Substitute for Counterstain Clearing

[Victoria Baker]

I think that it is called Crystal Mount - but apply to section, allow to
harden dip slide in clearing media and coverslip.  I know that there must
be others out there as well.
Good Luck



On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman <andrewcoleman131 <@t> gmail.com
> wrote:

> Hi all,
>
> We are performing a neutral red counterstain on tissue sections
> containing colored polystyrene microspheres. The spheres are inert to
> alcohol, but are washed out when we clear with xylene to coverslip.
> The spheres are also supposedly soluble in DMF, acetone, acetonitrile,
> chloroform and methylene chloride for what its worth.
>
> Is it reasonable to coverslip these slides in permanent mount without
> clearing with xylene after dehydrating the tissue? Or does anyone know
> of a substitute clearing agent with chemical properties dissimilar
> enough from xylene that might be worth trying instead?
>
> Thanks,
>
> Andrew
>
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