[Histonet] RE: IHC Validation

[Morken, Timothy]

All you need to do is compare the new clone to your current clone and show the new clone is concordant with the new clone for sensitivity and specificity . You don't need to compare to the same clone elsewhere.


Tim Morken
Department of Pathology
UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94132

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.morken <@t> ucsfmedctr.org




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J
Sent: Friday, May 04, 2012 5:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation

Hello all,
 
A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra.
For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT.  Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison.  
 
I'm now faced with changing clones for several antibodies.  I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours.  I suppose this really wouldn't be a 'true' validation in this case.
 
Does anyone have any thoughts on this?  The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone.  Any thoughts on how to perform a good validation in this case?
 
Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

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