[Histonet] IHC Validation

[burke]

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Dessoye,
Michael J
Sent: Friday, May 04, 2012 7:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation


Hello all,
 
A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra.
For validation, we selected a variety of cases as usual and ran them on
both instruments before we retired the XT.  Now, when we add a brand new
antibody, we again select a variety of cases, and once we're happy with
them on the Ultra we send the same cases to a reference lab for
comparison.  
 
I'm now faced with changing clones for several antibodies.  I expected
to go through pretty much the same validation procedure, but it got me
thinking...the reference lab does not always use the same clone as some
of ours.  I suppose this really wouldn't be a 'true' validation in this
case.
 
Does anyone have any thoughts on this?  The pathologists are perfectly
happy with the staining of the new clone, but the only reference lab I
can use uses a different clone.  Any thoughts on how to perform a good
validation in this case?
 
Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
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