[Histonet] RE: IHC Validation

[Dessoye, Michael J]

Thank you all for the info.  I agree that in-house validation is the way
to go.  The only reason we had been using an outside reference lab was
due to recommendations during a JC inspection which were applied to our
area, but not really meant for IHC.  The inspector was not a histology
person, so it was a little difficult to get our point across.  But they
were adamant that with only one instrument the slides should be sent out
for comparison.

Thanks everyone!

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 


-----Original Message-----
From: Morken, Timothy [mailto:Timothy.Morken <@t> ucsfmedctr.org] 
Sent: Friday, May 04, 2012 12:02 PM
To: Dessoye, Michael J; histonet <@t> lists.utsouthwestern.edu
Subject: RE: IHC Validation

All you need to do is compare the new clone to your current clone and
show the new clone is concordant with the new clone for sensitivity and
specificity . You don't need to compare to the same clone elsewhere.


Tim Morken
Department of Pathology
UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94132

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.morken <@t> ucsfmedctr.org




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dessoye,
Michael J
Sent: Friday, May 04, 2012 5:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation

Hello all,
 
A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra.
For validation, we selected a variety of cases as usual and ran them on
both instruments before we retired the XT.  Now, when we add a brand new
antibody, we again select a variety of cases, and once we're happy with
them on the Ultra we send the same cases to a reference lab for
comparison.  
 
I'm now faced with changing clones for several antibodies.  I expected
to go through pretty much the same validation procedure, but it got me
thinking...the reference lab does not always use the same clone as some
of ours.  I suppose this really wouldn't be a 'true' validation in this
case.
 
Does anyone have any thoughts on this?  The pathologists are perfectly
happy with the staining of the new clone, but the only reference lab I
can use uses a different clone.  Any thoughts on how to perform a good
validation in this case?
 
Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _
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