[Histonet] IHC Validation

[Kuhnla, Melissa]

Hi,
Think of yourself as a reference lab as well.  You have a validated
protocol for one clone already.  Validate the new clone against the
current. Your medical director can help you determine how many cases are
sufficient.  Depending on the antibody we validate anywhere from five
cases to twenty.
In my opinion, this in-house validation is the best way.  The only
variable is the clone.  Your processing, staining platform,
detection...all stays the same.
Good luck
melissa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dessoye,
Michael J
Sent: Friday, May 04, 2012 8:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Validation

Hello all,
 
A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra.
For validation, we selected a variety of cases as usual and ran them on
both instruments before we retired the XT.  Now, when we add a brand new
antibody, we again select a variety of cases, and once we're happy with
them on the Ultra we send the same cases to a reference lab for
comparison.  
 
I'm now faced with changing clones for several antibodies.  I expected
to go through pretty much the same validation procedure, but it got me
thinking...the reference lab does not always use the same clone as some
of ours.  I suppose this really wouldn't be a 'true' validation in this
case.
 
Does anyone have any thoughts on this?  The pathologists are perfectly
happy with the staining of the new clone, but the only reference lab I
can use uses a different clone.  Any thoughts on how to perform a good
validation in this case?
 
Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
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