[Histonet] Frozen brains on cryostat

[Andrea Ferullo (non-Celgene)]

Hello everyone,

I recently received rat brain samples that were frozen in liquid nitrogen and embedded in OCT.  I sectioned them on the cryostat and they are coming out very wrinkled, no matter what technique I use to pick them up.  I would appreciate any tips/tricks  that anyone has to offer.  Forebrain sections are ok, but mid, hind-brain, and cerebellum are giving me a very hard time.  Thanks and I look forward to your advice.

Andrea

*********************************************************
THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS
CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED
INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL
OR INDIVIDUALS NAMED ABOVE. 
If the reader is not the intended recipient, or the
employee or agent responsible to deliver it to the
intended recipient, you are hereby notified that any
dissemination, distribution or copying of this
communication is strictly prohibited. If you have
received this communication in error, please reply to the
sender to notify us of the error and delete the original
message. Thank You.
*********************************************************